Specificity:

Mouse Anti-Human CD2 (Clone G11) recognizes an epitope on Human CD2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

The CD2 antigen is present on approximately 80% of normal peripheral blood lymphocytes, >95% of thymocytes, 100% of mature circulating T-cells and a subset of NK cells.

Background:

Appropriate pairs of anti-CD2 antibodies will stimulate peripheral T-cell proliferation and effector function.¹ The CD2 molecule has been shown to be the receptor for leukocyte function antigen (LFA-3/CD58),² the binding of which augments T-cell activation mediated by the T-cell antigen receptor (TCR).³ Anti-Human CD2 can be used to deplete CD2 positive cells by complement-mediated cytotoxicity. Analyses of NK lymphocyte subsets and identification of lymphomas and leukemias of T-cell origin have been done using Anti-CD2 antibodies.

DyLight® 680 conjugated antibodies absorb light maximally around 682 nm (± 4 nm) and fluoresce maximally around 715 nm. DyLight® 680 produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5 and Alexa Fluor 680, and is ideal for multi-color applications including fluorescence microscopy, flow cytometry, ELISA. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+