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Anti-Mouse CD24 (CD24)
R-phycoerythrin (R-PE)
(Heat Stable Antigen, Nectadrin)
Monoclonal Antibody
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Prod. No.:C238
Clone:R7129.7
Isotype:Rat IgG
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD24 (Clone R7129.7) recognizes the cell surface glycoprotein Nectadrin, immunologically identical to the heat stable antigen (HSA). Nectadrin is a glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of Nectadrin purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kD.

Antigen Distribution:
The CD24 (Nectadrin) antigen is present on lymphocytes, granulocytes, erythrocytes.

Reporter Molecule:
R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.

Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 1 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Lesley, J. et al. (1988) Cell Immunol. 112:40
2. Kadmon, G. et al. (1992) J. Cell Biol. 118(5):1245
3. Alterman, L. A. et al. (1990) Eur. J. Immunol. 20(7):1597
4. Wegner, R. H. et al. (1993) J. Biol. Chem. 268:23345
Related Products
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Anti-Mouse CD24 (Heat Stable Antigen, Nectadrin)-Allophycocyanin (Leinco Prod. No.: C343)
PN:
Anti-Mouse CD24 (Heat Stable Antigen, Nectadrin) Purified (Leinco Prod. No.: C235)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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