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Anti-Mouse CD4 (CD4)
DyLight® 633
(CD4mut, L3T4, Ly-4, Cd4, CD4 Antigen)
Monoclonal Antibody
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Prod. No.:C1639
Clone:GK1.5
Isotype:Rat IgG2b
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse (CD4) (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.

Background:
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.1

Reporter Molecule:
DyLight® 633 antibody conjugates absorb light maximally around 638 nm (± 4 nm) and fluoresce with a peak around 658 nm. Antibodies conjugated to Dylight® 633 may be used in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and are ideal for multi-color applications. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Ms+
Host Species
Rat
Formulation
This DyLight® 633 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 633 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Hendrickson WA et al. (1994) Structure 2: 59
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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