Specificity:

Mouse Anti-Human CD9 (Clone MM2/57) recognizes Human CD9. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody reacts with a 25 kDa molecular weight band on gels of non-reduced platelet membranes and immunoprecipitates a similar band from Iodine 125 labeled platelets. The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals localized in the cytoplasm. This antibody reacts immunohistochemically on frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues.

R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.

Known Species Cross Reactivity: Ms+, Hu+, Rb+