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Anti-Human CXCL5
Purified No Carrier Protein
(AMCF-II, Epithelial-Derived Neutrophil-Activating Peptide 78 (ENA-78), GCP-2, Scyb5, Scyb6, Lipopolysaccharide-Induced CXC Chemokine (LIX))
Monoclonal Antibody
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Prod. No.:C1386
Clone:33160
Isotype:Mouse IgG1
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CXCL5 (Clone 33160) recognizes an epitope on Human CXCL5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
CXCL5 is an inflammatory chemokine that belongs to the CXC chemokine family1 that has a role in the development of cardiovascular diseases.2 CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils.1 It is expressed by epithelial cells within colorectal mucosa is a chemoattractant for neutrophils and has been implicated in Crohn's disease and ulcerative colitis.3 CXCL5 is important in growth and development of colorectal cancer, implicating a future role in both cancer therapy and diagnosis.4

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human CXCL5 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human CXCL5 is approximately 1.5 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 - 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human CXCL5 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL5 (at 30 ng/ml) is 3 - 15 µg/ml.
Western Blotting: To detect Human CXCL5 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CXCL5 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human CXCL5 (Leinco Prod. No.: E109).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Rajashekhar G et al. (2007) Physiol Genomics 31: 104
2. Mahsa M et al. (2008) Molec Diagnosis & Therapy 12: 391
3. Wågsäter Dick et al. (2007) International J Oncol. 31: 97
4. Fred Nagelkerke et al. (2008) Clinical Cancer Research 14: 2276
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human CXCL5 (Leinco Prod. No.: E109)
Anti-Mouse CXCL5 (Antigen Aff. Pur.) (Leinco Prod. No.: L138)
Anti-Human CXCL5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E122)
Recombinant Mouse CXCL5 (Leinco Prod. No.: L123)
Anti-Rat CXCL5 (Leinco Prod. No.: L217)
Anti-Rat CXCL5 (Antigen Aff. Pur.) (Leinco Prod. No.: L221)
Anti-Rat CXCL5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L231)
Anti-Human CXCL5 (Antigen Aff. Pur.) (Leinco Prod. No.: E106)
Anti-Mouse CXCL5 (Leinco Prod. No.: C1414)
Anti-Human CXCL5 (Leinco Prod. No.: C1607)
Anti-Mouse CXCL5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L140)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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