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Anti-Human CXCL5
Purified No Carrier Protein
(ENA-78)
Monoclonal Antibody
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Prod. No.:C1607
Clone:33170
Isotype:Mouse IgG1
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CXCL5 (Clone 33170) recognizes an epitope on Human CXCL5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human CXCL5 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, FC, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is Anti-Human CXCL5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E122). A suggested standard for this assay is Recombinant Human CXCL5 (Leinco Prod. No.: E109).
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCL5. A suggested method would be to stain cells expressing CXCL5 with 25 µg per 1-5x 105 cells in a total staining volume of 200 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Neutralization: This antibody is useful for neutralization of Human CXCL5 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CXCL5 (at 30 ng/ml) is 3-12 µg/ml.
Western Blotting: To detect Human CXCL5 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human CXCL5 is 20 ng/lane under non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human CXCL5 (Leinco Prod. No.: E109).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Anti-Human CXCL5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E122)
Recombinant Human CXCL5 (Leinco Prod. No.: E109)
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse CXCL5 (Antigen Aff. Pur.) (Leinco Prod. No.: L138)
Recombinant Mouse CXCL5 (Leinco Prod. No.: L123)
Anti-Rat CXCL5 (Leinco Prod. No.: L217)
Anti-Rat CXCL5 (Antigen Aff. Pur.) (Leinco Prod. No.: L221)
Anti-Rat CXCL5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L231)
Anti-Human CXCL5 (Antigen Aff. Pur.) (Leinco Prod. No.: E106)
Anti-Human CXCL5 (Leinco Prod. No.: C1386)
Anti-Mouse CXCL5 (Leinco Prod. No.: C1414)
Anti-Mouse CXCL5-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: L140)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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