Specificity:

Mouse Anti-Human Caspase-3 Active Subunit (p17) (CASP3) (Clone C31.129) recognizes an epitope on Human CASP3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:

Caspase-3 is a member of the caspase family of cysteine proteases and plays a key role in mediating Fas (CD95) and tumor necrosis factor–1 (TNFR-1) induced apoptosis. Caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators.^1,2 Caspase-3 is activated during apoptotic signaling events by upstream proteases including caspase 6 and caspase 8.³ Caspase-3 is thought to be essential for chromatin margination, DNA fragmentation and nuclear collapse during apoptosis.³

DyLight® 594 antibody conjugates absorb light maximally around 593 nm (± 4 nm) and fluoresce with a peak around 618 nm. They are distinctly brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+