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Anti-Mouse Chemokine (C-C Motif) Ligand 1 (CCL1)
Purified No Carrier Protein
(SCYA1, I-309, T Cell Activation Gene 3 (TCA3), P500, SISe)
Monoclonal Antibody
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Prod. No.:T458
Clone:148113
Isotype:Rat IgG2a
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse Chemokine (C-C Motif) Ligand 1 (CCL1) (Clone 148113) recognizes an epitope on Mouse CCL1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.1 CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.2 CCL1 may play a role in lymphocyte recruitment in bronchial asthma.3

Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse TCA-3
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380). The detection sensitivity of this indirect ELISA for Mouse TCA-3 is approximately 5 ng/well.
Neutralization: This antibody is useful for neutralization of Mouse TCA-3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse TCA-3 (at 10 ng/ml) is 2-10 µg/ml.
Western Blotting: To detect Mouse TCA-3 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for rmTCA-3 is approximately 25 ng/lane and 100 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Krangel et al. (1992) Proceedings of the National Acad of Sci. 89: 2950
2. Roos et al. (1997) J Biol Chem. 272: 17251
3. Teran LM et al. (2006) Eur Respir J. 28: 59
Related Products
Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380)
Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: I-231)
Anti-Mouse CCL1 (Leinco Prod. No.: T459)
Anti-Mouse CCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: C1483)
Anti-Mouse CCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T504)
Anti-Human CCL1 (Leinco Prod. No.: I-643)
Anti-Human CCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-177)
Recombinant Human CCL1 (Leinco Prod. No.: I-224)
Recombinant Mouse CCL1 (Leinco Prod. No.: T410)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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