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Anti-Mouse Chemokine (C-C Motif) Ligand 27 (CCL27)
Purified No Carrier Protein
(ALP, Eskine, Cutaneous T-Cell Attracting Chemokine, CTACK, SCYA27, ILC, skinkine, PESKY)
Monoclonal Antibody
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Prod. No.:C1452
Clone:68623
Isotype:Rat IgG2b
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse Chemokine (C-C Motif) Ligand 27 (CCL27) (Clone 68623) recognizes an epitope on Mouse CCL27. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family. It is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediated inflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotactic effects by binding to the chemokine receptor CCR10.

CCL27 is one of the CC chemokines produced by epidermal keratinocytes and is suggested to be involved in the pathogenesis of inflammatory skin diseases. CCL27 modifies inflammation by attracting CCR10-positive and CCR4-positive cells into the lesional skin, and may participate in the pathogenesis of Th2-shifted skin diseases such as atopic dermatitis.

Known Species Cross Reactivity: Ms+
Host Species
Rat
Immunogen
Purified Recombinant Mouse CCL27 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2 µg/ml. A suitable detection antibody is Anti-Mouse CCL27-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1520) at a concentration of approximately 200 ng/ml. A suggested standard for this assay is Recombinant Mouse CCL27 (Leinco Prod. No.: C359).
Neutralization: This antibody is useful for neutralization of Mouse CTACK bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CTACK (at 1.0 ng/ml) is 2.5 - 10.0 µg/ml.
Western Blotting: To detect Mouse CTACK this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse CTACK is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Anti-Mouse CCL27-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1520)
Recombinant Mouse CCL27 (Leinco Prod. No.: C359)
Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CCL27 (Antigen Aff. Pur.) (Leinco Prod. No.: C1376)
Anti-Human CCL27 (Leinco Prod. No.: C1409)
Anti-Human CCL27 (Leinco Prod. No.: C1410)
Anti-Mouse CCL27/CTACK (Leinco Prod. No.: C1453)
Anti-Mouse CCL27/CTACK (Antigen Aff. Pur.) (Leinco Prod. No.: C1475)
Anti-Human CCL27/CTACK-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1494)
Recombinant Human CCL27 (Leinco Prod. No.: C841)
Anti-Human CCL27 (Leinco Prod. No.: C1605)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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