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Anti-Mouse Chemokine (C-X-C Motif) Ligand 2 (CXCL2)
Purified No Carrier Protein
(SCYB2, GRO2, Growth-Regulated Protein Beta (GROb), MIP-2a, MGSA-b, CINC-2a, MIP-2, Cytokine-Induced Neutrophil Chemotactic Factor 3 (CINC-3))
Monoclonal Antibody
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Prod. No.:M1277
Clone:40605
Isotype:Rat IgG2b
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Clone 40605) recognizes an epitope on Mouse CXCL2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Chemokine (C-X-C motif) ligand 2 (CXCL2) is an inducible murine chemokine involved in attraction of polymorphonuclear granulocytes to sites of infection.1 This chemokine is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells.2,3,4

Known Species Cross Reactivity: Ms+
Host Species
Rat
Immunogen
Purified Recombinant Mouse CXCL2 (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, N, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.1-0.4 µg/ml. A suitable detection antibody is Anti-Mouse CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M195) at a concentration of approximately 2.0 µg/ml. When used in conjunction with an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118), the sensitivity of this sandwich ELISA is 2 ng/well. A suggested standard for this assay is Recombinant Mouse MIP-2 (Leinco Prod. No.: M174).
Neutralization: This antibody is useful for neutralization of Mouse MIP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse MIP-2 (at 2 ng/ml) is 1-4 µg/ml.
Western Blotting: To detect Mouse CXCL2 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse CXCL2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse MIP-2 (Leinco Prod. No.: M174).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. H. Ulrich Beuscher et al. (2004) International Immunol. 16: 1675
2. Cerami A et al. (1989) Proc Nat Acad Sci. 86: 612
3. Grotendorst G.R et al. (1990) Mol Cell Biol. 10: 5596
4. Fukuda S et al. (2006) Exp Hematol. 34: 1010
Related Products
Anti-Mouse CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M195)
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" (Leinco Prod. No.: T118)
Recombinant Mouse MIP-2 (Leinco Prod. No.: M174)
Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G146)
Anti-Human CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G148)
Anti-Rat CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: G122)
Anti-Mouse CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: M178)
Anti-Human CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: G133)
Mouse CXCL2 ELISA Development Kit (Leinco Prod. No.: M1108)
Recombinant Mouse CXCL2 (Leinco Prod. No.: G632)
Anti-Human CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: G136)
Human GROβ (CXCL2) ELISA Development Kit (Leinco Prod. No.: G595)
Recombinant Rat CXCL2 (Leinco Prod. No.: C1343)
Anti-Rat CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: C1465)
Anti-Rat CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1509)
Anti-Rat CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G147)
Anti-Rat CXCL2 (Leinco Prod. No.: C1433)
Recombinant Human CXCL2 (Leinco Prod. No.: G131)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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