Product Datasheet
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Anti-Human Fibronectin
Purified
Monoclonal Antibody
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Size


Prod. No.:F1102
Clone:HFN 7.1
Isotype:Mouse IgG1
Conc.:0.2 mg/ml
Pkg. Size:50 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Fibronectin Ab-1 (Clone HFN 7.1) is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-mediated cell attachment not by disrupting the collagen-fibronectin interaction but by interfering with the attachment of fibronectin to its receptor on the cell surface.

Antigen Distribution:
Fibronectin is a dimeric glycoprotein which is present in cells, extracellular matrix and blood.

Host Species
Mouse
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IP, WB, B
Immunoprecipitation: 2 µg/mg of protein lysate
Western Blotting: To detect Human Fibronectin this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human Fibronectin (Leinco Prod. No.: F105)
Anti-Matrix Fibronectin (Leinco Prod. No.: F1101)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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