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Anti-Human Flt-3 Ligand (FLT3LG)
Purified No Carrier Protein
(Fms-Related Tyrosine Kinase 3 Ligand, Flk-2 Ligand, FL, FLT3LG, STK-1 Ligand)
Monoclonal Antibody
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Prod. No.:F1071
Clone:40406
Isotype:Mouse IgG1
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Flt-3 Ligand (Clone 40406) recognizes an epitope on Human Flt-3 Ligand. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Fms-related tyrosine kinase 3 ligand (FLT3LG) is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, such as IL-7 and IL-3, Flt-3 ligand promotes long-term expansion and differentiation of human pro-B-cells.1,2 Flt3 ligand is a cytokine capable of inducing large numbers of dendritic cells that dramatically enhances the sensitivity of antigen-specific B and T cell responses to systemic injection of a soluble protein. Through a CD40-CD40 ligand-dependent mechanism Flt-3 Ligand influences the class of antibody produced and enables productive immune responses.3

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human Flt-3 Ligand (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human Flt-3 Ligand is approximately 3.0 ng/well.
Neutralization: This antibody is useful for neutralization of Human Flt-3 Ligand bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human Flt-3 Ligand (at 5.0 ng/ml) is 0.02 - 0.06 µg/ml.
Western Blotting: To detect Human Flt-3 Ligand this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human Flt-3 Ligand is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human Flt-3 (Leinco Prod. No.: F1031).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Mattson J et al. (1994) Nature 368: 643
2. Cleveland LS et al. (1995) Oncogene 10: 149
3. C.R. Maliszewski et al. (1998) J. Exp. Med. 188: 2075
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human Flt-3 (Leinco Prod. No.: F1031)
Anti-Mouse Flt-3 Ligand (Leinco Prod. No.: F1073)
Anti-Human Flt-3 Ligand (Antigen Aff. Pur.) (Leinco Prod. No.: F108)
Anti-Mouse Flt-3 Ligand (Antigen Aff. Pur.) (Leinco Prod. No.: F1062)
Anti-Mouse Flt-3 Ligand-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: F1096)
Recombinant Human Flt-3 Ligand (Leinco Prod. No.: F114)
Recombinant Mouse Flt-3 Ligand (Leinco Prod. No.: F159)
Anti-Human Flt-3 Ligand-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: F135)
Anti-Human Flt-3 Ligand (Leinco Prod. No.: F1076)
Recombinant Human Flt-3 Ligand (Leinco Prod. No.: F1029)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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