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Anti-Human HLA-DP Monomorphic (HLA-DP)
DyLight® 550
Monoclonal Antibody
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Prod. No.:H1583
Clone:B7/21
Isotype:Mouse IgG3
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human HLA-DP Monomorphic (HLA-DP) (Clone B7/21) recognizes an epitope on Human HLA-DP. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The HLA-DP antigen is present on approximately 10% of normal peripheral blood lymphocytes but not on resting peripheral T-lymphocytes. HLA-DP is present in low density on peripheral blood monocytes and mitogen-stimulated T-lymphoblasts. Anti-HLA-DP reacts with most B-cell lines.

Reporter Molecule:
DyLight® 550 conjugated antibodies absorb light maximally around 562 (± 4 nm) and fluoresce with a peak around 576 nm. They are brighter than TRITC conjugates, and they are equal in brightness to Cy3 and Alexa Fluor 555. All Dylight® 550 antibodies are recommended for maximum sensitivity in all immunofluorescence detection procedures within the orange-red portion of the visible spectrum. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human HLA-DP (>98%)
Formulation
This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Watson, J. A. et al. (1983) Nature 304:358
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