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Anti-Mouse Insulin-Like Growth Factor Binding Protein 2 (IGFBP-2)
Purified No Carrier Protein
(IBP-2,IGF-BP53)
Monoclonal Antibody
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Prod. No.:I-711
Clone:150101
Isotype:Rat IgG2a
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse Insulin-Like Growth Factor Binding Protein 2 (IGFBP-2) (Clone 150101) recognizes an epitope on Mouse IGFBP-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Insulin-like growth factor binding protein 2, also known as IGFBP2, is an extracellular unglycosylated polypeptide that specifically binds IGF and modulates its effects.1 IGFBP-2 has direct effects on breast cancer proliferation via stimulation of critical signaling pathways.2

Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse IGFBP-2
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380). The detection sensitivity of this indirect ELISA for Mouse IGFBP-2 is approximately 5 ng/well.
Neutralization: This antibody is useful for neutralization of Mouse IGFBP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IGFBP-2 (at 0.75 ng/ml) is 5 - 20 µg/ml.
Western Blotting: To detect Mouse IGFBP-2 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse IGFBP-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Luthman H. et al.(1991) Biochem Biophys Res Commun. 176: 1250
2. Disis ML. et al.(2008) Cancer Res. 68: 8400
Related Products
Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380)
Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse IGFBP-2 (Leinco Prod. No.: I-588)
Anti-Human IGFBP-2 (Leinco Prod. No.: I-693)
Anti-Human IGFBP-2 (Leinco Prod. No.: I-694)
Anti-Human IGFBP-2 (Antigen Aff. Pur.) (Leinco Prod. No.: I-731)
Anti-Mouse IGFBP-2 (Antigen Aff. Pur.) (Leinco Prod. No.: I-742)
Anti-Human IGFBP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-791)
Anti-Mouse IGFBP-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-801)
Recombinant Human IGFBP-2 (Leinco Prod. No.: I-375)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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