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Anti-Human Insulin-Like Growth Factor I (IGF-I)
Purified No Carrier Protein
(Somatamedin C, IGF-IA )
Monoclonal Antibody
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Prod. No.:I-649
Clone:56408
Isotype:Mouse IgG1
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Insulin-Like Growth Factor I (IGF-I) (Clone 56408) recognizes an epitope on Human IGF-I. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
The IGFs are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human IGF-I (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, IHC (NBF/Par.), WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is Anti-Human IGF-I-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-235) at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is Recombinant Human IGF-I (Leinco Prod. No.: I-180).
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human IGF-I this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IGF-I is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human IGF-I (Leinco Prod. No.: I-180).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Anti-Human IGF-I-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-235)
Recombinant Human IGF-I (Leinco Prod. No.: I-180)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse IGF-I (Leinco Prod. No.: I-239)
PN:
Anti-Mouse IGF-I (Antigen Aff. Pur.) (Leinco Prod. No.: I-340)
Anti-Mouse IGF-I-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-240)
Anti-Mouse IGF-I (Leinco Prod. No.: I-709)
Recombinant Human IGF-I R (Leinco Prod. No.: I-841)
Recombinant Rat IGF-I (Leinco Prod. No.: I-524)
Anti-Human IGF-I (Antigen Aff. Pur.) (Leinco Prod. No.: I-142)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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