Product Datasheet
www.leinco.com
excellence in early discovery research       



Anti-Human Inter-Cellular Adhesion Molecule 1 (ICAM-1)
Allophycocyanin (APC)
(BB2, CD54, P3.58)
Monoclonal Antibody
Share |


Prod. No.:C323
Clone:15.2
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Description
Specificity:
Mouse Anti-Human Inter-Cellular Adhesion Molecule 1 (ICAM-1) (Clone 15.2) recognizes an epitope on Human ICAM-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
ICAM-1 is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. CD54 is the receptor for rhinoviruses and malaria.1

Background:
Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein1 and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines.2 ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies.3

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human ICAM-1 (>98%)
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Li S et al. (2009) Biochem Biophys Res Commun. 381: 459
2. Wolf S et al. (2009) Pharmacol Rep 61: 22
3. Ozcan U et al. (2009) Arch Gynecol Obstet.
Related Products
Anti-Human ICAM-1 Purified (Leinco Prod. No.: C167)
Anti-Human ICAM-1-Fluorescein (FITC) (Leinco Prod. No.: C169)
Anti-Human ICAM-1-R-phycoerythrin/Cy5 (Leinco Prod. No.: C346)
Recombinant Rat ICAM-1 (Leinco Prod. No.: I-571)
Anti-Rat ICAM-1 (Leinco Prod. No.: I-682)
Anti-Human ICAM-1 (Leinco Prod. No.: I-698)
Anti-Rat ICAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: I-727)
Anti-Human ICAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: I-734)
Anti-Mouse ICAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: I-741)
Anti-Rat ICAM-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-786)
Anti-Human ICAM-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-794)
Recombinant Human ICAM-1 (Leinco Prod. No.: I-580)
Recombinant Mouse ICAM-1 (Leinco Prod. No.: I-587)
Anti-Mouse ICAM-1-Allophycocyanin (Leinco Prod. No.: C354)
Anti-Mouse ICAM-1-Fluorescein (Leinco Prod. No.: C269)
Anti-Mouse ICAM-1-Biotin (Leinco Prod. No.: C268)
Anti-Mouse ICAM-1-R-phycoerythrin (Leinco Prod. No.: C270)
Anti-Human ICAM-1 Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C372)
Anti-Mouse ICAM-1 Low Endotoxin Functional Formulation (Leinco Prod. No.: C391)
Anti-Human ICAM-1-Biotin (Leinco Prod. No.: C168)
PN:
Anti-Human ICAM-1-R-phycoerythrin (R-PE) (Leinco Prod. No.: C170)
Anti-Mouse ICAM-1 Purified (Leinco Prod. No.: C267)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2013