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Anti-Human Interferon Gamma (IFN-γ)
Purified No Carrier Protein
(Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI)
Monoclonal Antibody
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Prod. No.:I-646
Clone:25718
Isotype:Mouse IgG2a
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Interferon Gamma (IFN-γ) (Clone 25718) recognizes an epitope on Human IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human IFN-γ (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human IFN-γ is approximately 25 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human IFN-γ bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IFN-γ (at 50 ng/ml) is 0.02 - 0.06 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Goeddel DV et al. (1982) Nature 298: 859
2. Wilson CB et al. (2007) Adv. Immunol. 96: 41
3. Hume DA et al. (2004) J Leukoc Biol. 75: 163
Related Products
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
Anti-Mouse IFN-γ-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-201)
Recombinant Human IFN-γ (Leinco Prod. No.: I-179)
Recombinant Mouse IFN-γ (Leinco Prod. No.: I-217)
Anti-Rat IFN-γ (Antigen Aff. Pur.) (Leinco Prod. No.: I-233)
Anti-Human IFN-γ (Leinco Prod. No.: I-328)
Anti-Human IFN-γ (Antigen Aff. Pur.) (Leinco Prod. No.: I-141)
Anti-Human IFN-γ (Leinco Prod. No.: I-647)
Anti-Mouse IFN (Leinco Prod. No.: I-669)
Anti-Rat IFN-γ (Leinco Prod. No.: I-683)
Mouse IFN-γ ELISA Development Kit (Leinco Prod. No.: I-297)
Rat IFN-γ ELISA Development Kit (Leinco Prod. No.: I-381)
Anti-Mouse IFN-γ (Antigen Aff. Pur.) (Leinco Prod. No.: I-232)
Anti-Human IFN-γ-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-178)
Anti-Rat IFN-γ-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-203)
Recombinant Rat IFN-γ (Leinco Prod. No.: I-223)
Human IFN-γ ELISA Development Kit (Leinco Prod. No.: I-296)
Anti-Mouse IFN-γ Low Endotoxin Functional Formulation (Leinco Prod. No.: I-438)
PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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