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Anti-Human Interleukin-1 Receptor Type I (IL-1 RI)
Purified No Carrier Protein
Monoclonal Antibody
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Prod. No.:I-642
Clone:35730
Isotype:Mouse IgG1
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human Interleukin 1 Receptor Type I (IL-1 RI) (Clone 35730) recognizes an epitope on Human IL-1 RI. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. No cross-reactivity was observed with rhIL-1 RII, rhIL-1 R5 or rmIL-1 RI.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human IL-1 RI (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Cap, WB
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is Anti-Human IL-1 RI-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-766) at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is Recombinant Human IL-1 RI (Leinco Prod. No.: I-556).
Western Blotting: To detect Human IL-1 RI this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-1 RI is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human IL-1 RI (Leinco Prod. No.: I-556).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Anti-Human IL-1 RI-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-766)
Recombinant Human IL-1 RI (Leinco Prod. No.: I-556)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse IL-1 RI (Leinco Prod. No.: I-582)
Anti-Mouse IL-1 R1 (Leinco Prod. No.: I-704)
Anti-Mouse IL-1 RI (Antigen Aff. Pur.) (Leinco Prod. No.: I-736)
Anti-Mouse IL-1 RI-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-796)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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