Anti-MAP-2 – DyLight® 488

Bovine brain microtubule associated protein 2

This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This DyLight^® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Next Day 2-8°C

Blue Laser (493 nm)

Mouse Anti-Human MAP-2 (Clone AP20) recognizes the epitope to aa 997-1332 on Human MAP-2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

This antibody is specific to MAP2a and MAP2b, and will not cross-react with MAP1, MAP5, tubulin or tau. This clone reacts with dendrites and cell bodies of neurons but not with the neuronal processes. It does not react with the low MW form (70 kDa, MAP2c).

Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau). MAP2 isoforms are expressed only in neuronal cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. MAP2 serves to stabilize microtubules (MT) growth by crosslinking MT with intermediate filaments and other MT.

1. Cacares, A. et al. (1984) J. Neuroscience 4:394 2. Peng, I. et al. (1986) J. Cell Biol. 102:252 3. Lewis, S. A. et al. (1989) Nature 342:498