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Anti-Rat PCNA (PCNA)
Fluorescein (FITC)
Monoclonal Antibody
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Prod. No.:P105
Clone:PC10
Isotype:Mouse IgG2a κ
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Anti-PCNA (Proliferating Cell Nuclear Antigen) recognizes polymerase delta accessory protein (Mr 36 kDa).1,3 This auxiliary protein is also known as cyclin and experiments strongly suggest that PCNA is essential for cellular DNA synthesis.2,3

Antigen Distribution:
Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both. Dominant PCNA expression begins during late G1 phase and is present through S phase of the cell cycle.

Reporter Molecule:
Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.

Known Species Cross Reactivity: Ms+, Hu+, Rt+, All vertebrates and invertebrates
Host Species
Mouse
Immunogen
Rat PCNA made in the protein A vector pR1T2T
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 0.125 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
References
1. Tan, C. et al. (1987) Nucleic Acids Research 15:9299
2. Zuber, M. et al. (1989) Molec. Cell. Biol. 9:57
3. Waseem, H. N. et al. (1990) J. Cell Sci. 96:121
Related Products
Anti-PCNA Biotin (Leinco Prod. No.: P104)
Anti-PCNA Allophycocyanin (Leinco Prod. No.: P120)
Anti-PCNA Purified (Leinco Prod. No.: P103)
Anti-PCNA R-phycoerythrin (Leinco Prod. No.: P106)
PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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