Mouse Anti-Rat PCNA (Clone PC10) recognizes an epitope on Rat PCNA. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both. Dominant PCNA expression begins during late G1 phase and is present through S phase of the cell cycle.
DyLight® 680 conjugated antibodies absorb light maximally around 682 nm (± 4 nm) and fluoresce maximally around 715 nm. DyLight® 680 produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5 and Alexa Fluor 680, and is ideal for multi-color applications including fluorescence microscopy, flow cytometry, ELISA.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Known Species Cross Reactivity: Rt+
Purified Recombinant Rat PCNA (>98%)
This DyLight® 680 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 680 conjugate is stable when stored at 2-8°C. Do not freeze.
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
1. Tan, C. et al. (1987) Nucleic Acids Research 15:9299
2. Zuber, M. et al. (1989) Molec. Cell. Biol. 9:57
3. Waseem, H. N. et al. (1990) J. Cell Sci. 96:121