Anti-Human Platelet-Derived Growth Factor B (PDGF-B)
Purified No Carrier Protein
Monoclonal Antibody
| Prod. No.: | P272 |
| Clone: | 108132 |
| Isotype: | Mouse IgG1 |
| Pkg. Size: | 1 mg, 500 µg |
| Storage: | -20°C to -70°C Detailed storage instructions below. |
Description
Specificity:
Mouse Anti-Human Platelet-Derived Growth Factor B (PDGF-B) (Clone 108132) recognizes an epitope on Human PDGF-B. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. In direct ELISAs, this antibody shows approximately 40% cross-reactivity with rhPDGF-AB and no cross-reactivity with rhCTGF, rhLDGF, rhPDGF-AA, rrPDGF-AA, rrPDGF-AB, rrPDGF-BB, rhPDGF-C, rhPDGF-D, rhPlGF, rhVEGF, rhVEGF-C, or rhVEGF-D
Background:
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB
Known Species Cross Reactivity: Hu+
Host Species
Mouse
Immunogen
Purified Recombinant Human PDGF-B (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (
Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human PDGF-B is approximately 1 ng/well.
Western Blotting: To detect Human PDGF-B this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (
Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (
Leinco Prod. No.: T343). The detection limit for Human PDGF-B is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human PDGF-BB (
Leinco Prod. No.: P126).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
USA
Related Products
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
