Product Datasheet
www.leinco.com
excellence in early discovery research       



Anti-Mouse Vγ 3 T-Cell Receptor (TCR)
DyLight® 488
Monoclonal Antibody
Share |


Prod. No.:T566
Clone:536
Isotype:Syrian Hamster IgG
Conc.:0.5 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Syrian Hamster Anti-Mouse Vγ 3 T-Cell Receptor (TCR) (Clone 536) recognizes an epitope on Mouse TCR. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The antibody 536 stained less than 1% of cells in adult thymus (AKR), splenocytes, lymph node, adult CD4-8- thymocytes, and adult CD4-8-, T splenocyte. 92% of day 14 old BALB/C fetal thymus, 86% of C57BL/6 dEC (Thy-1+) and 94% of AKR dEC (Thy-1+) stained positive using this antibody.

Background:
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

Reporter Molecule:
DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Ms+
Host Species
Syrian Hamster
Immunogen
Purified Recombinant Mouse TCR (>98%)
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Havran, et al. (1989) Pro. Natl. Acad. Sci. USA 86:4185
Related Products
Anti-Mouse Vγ 3 T-Cell Receptor (TCR)-Fluorescein (Leinco Prod. No.: T135)
Anti-Mouse Vγ 3 T-Cell Receptor (TCR)-R-phycoerythrin (Leinco Prod. No.: T136)
Anti-Mouse Vγ 3 T-Cell Receptor (TCR)-Allophycocyanin (Leinco Prod. No.: T144)
Anti-Mouse Vγ 2 T-Cell Receptor (TCR)-Fluorescein (Leinco Prod. No.: T212)
Anti-Mouse Vγ 2 T-Cell Receptor (TCR)-Low Endotoxin Functional Formulation (Leinco Prod. No.: T353)
Anti-Mouse Vγ 3 T-Cell Receptor (TCR)-Purified (Leinco Prod. No.: T133)
Anti-Mouse Vγ 3 T-Cell Receptor (TCR)-Biotin (Leinco Prod. No.: T134)
Anti-Mouse Vγ 2 T-Cell Receptor (TCR)-Purified (Leinco Prod. No.: T210)
PN:
Anti-Mouse Vγ 2 T-Cell Receptor (TCR)-Allophycocyanin (Leinco Prod. No.: T219)
Anti-Mouse Vγ 2 T-Cell Receptor (TCR)-R-phycoerythrin (Leinco Prod. No.: T213)
Anti-Mouse γ/δ T-Cell Receptor (TCR)-Allophycocyanin (Leinco Prod. No.: T143)
Anti-Mouse γ/δ T-Cell Receptor (TCR)-R-phycoerythrin (Leinco Prod. No.: T132)
Anti-Mouse γ/δ T-Cell Receptor (TCR)-Fluorescein (Leinco Prod. No.: T131)
Anti-Mouse γ/δ T-Cell Receptor (TCR)-Purified (Leinco Prod. No.: T129)
Anti-Mouse Vγ 2 T-Cell Receptor (TCR)-Biotin (Leinco Prod. No.: T211)
Anti-Mouse γ/δ T-Cell Receptor (TCR)-Functional Formulation (Leinco Prod. No.: T352)
Anti-Mouse γ/δ T-Cell Receptor (TCR)-Biotin (Leinco Prod. No.: T130)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2013