Specificity:

Mouse Anti-Human p34^cdc2 Serine/Threonine Kinase (Clone 17) recognizes an epitope on Human p34^cdc2 Serine/Threonine Kinase. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:

p34^cdc2 is thought to be activated by cyclin through dephosphorylation at tyrosine residues. Activated p34^cdc2 kinase is involved in a number of mitotic events such as nuclear envelope breakdown and chromosome condensation through phosphorylation of serine/threonine residues of substrates such as nuclear lamins, histones, pp60c-src, nucleolin and RNA polymerase II. It is then inactivated by a tyrosine kinase. Anti-p34^cdc2 can be used in western blotting, immunoprecipitation and immunohistochemistry. The antibody precipitates an active kinase.

DyLight® 488 conjugated antibodies absorb light maximally around 493 nm (± 4 nm) and fluoresce with a peak around 518 nm. They are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates, but typically give less background. DyLight® 488 conjugated to any antibody is recommended to maximize sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+