Specificity:

Mouse Anti-Human p34^cdc2 Serine/Threonine Kinase (Clone 17) recognizes an epitope on Human p34^cdc2 Serine/Threonine Kinase. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:

p34^cdc2 is thought to be activated by cyclin through dephosphorylation at tyrosine residues. Activated p34^cdc2 kinase is involved in a number of mitotic events such as nuclear envelope breakdown and chromosome condensation through phosphorylation of serine/threonine residues of substrates such as nuclear lamins, histones, pp60c-src, nucleolin and RNA polymerase II. It is then inactivated by a tyrosine kinase. Anti-p34^cdc2 can be used in western blotting, immunoprecipitation and immunohistochemistry. The antibody precipitates an active kinase.

DyLight® 550 conjugated antibodies absorb light maximally around 562 (± 4 nm) and fluoresce with a peak around 576 nm. They are brighter than TRITC conjugates, and they are equal in brightness to Cy3 and Alexa Fluor 555. All Dylight® 550 antibodies are recommended for maximum sensitivity in all immunofluorescence detection procedures within the orange-red portion of the visible spectrum. DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Known Species Cross Reactivity: Hu+