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Goat Anti-Human A Proliferating-Inducing Ligand (APRIL)
Antigen Affinity Purified
(CD256, TNFSF13, TALL2, TRDL1)
Polyclonal Antibody
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Prod. No.:A206
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human A Proliferating-Inducing Ligand (APRIL) recognizes Human APRIL. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
A proliferation-inducing ligand (APRIL) is a TNF-like cytokine that stimulates tumor cell growth.1 It is of low abundance in normal tissues.2 APRIL acts as a proliferative factor in megakaryocytopoiesis, a developmental hematopoietic process responsible for progenitor cell differentiation to megakaryoblasts and megakaryocytes, leading to platelet formation.3 APRIL may be implicated in the regulation of tumor cell growth.2

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human APRIL (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, FC, WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Sarah G. Hymowitz et al. (2004) J Mol Biol. 343: 283
2. (1998) Jürg Tschopp
3. Ruggero De Maria et al. (2004) Blood 104: 3169
Related Products
Anti-Human APRIL-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: A207)
APRIL (Extracellular Domain-2) Blocking Peptide (Leinco Prod. No.: A186)
Anti-Human APRIL (Extracellular Domain-1) (Leinco Prod. No.: A154)
Anti-Human APRIL (Extracellular Domain-2) (Leinco Prod. No.: A155)
APRIL (Extracellular Domain-1) Blocking Peptide (Leinco Prod. No.: A185)
Recombinant Mouse APRIL (A Proliferation-Inducing Ligand) (Leinco Prod. No.: A200)
Recombinant Human APRIL (Leinco Prod. No.: A413)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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