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Rabbit Anti-Human Acinus (ACIN1)
Purified No Carrier Protein
(Apoptotic Chromatin Condensation Inducer in the Nucleus)
Polyclonal Antibody
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Prod. No.:A148
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human Acinus (CT-2) recognizes an epitope near the C-terminus of Human Acinus (CT-2). This polyclonal antibody was purified using immobilized peptide affinity chromatography.

Background:
Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’.2

Known Species Cross Reactivity: Hu+
Immunogen
Acinus (C-Terminus 2) Blocking Peptide (Leinco Prod. No.: A166)
Blocking Peptide
Acinus (C-Terminus 2) Blocking Peptide (Leinco Prod. No.: A166)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.

2. Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
Related Products
Acinus (C-Terminus 2) Blocking Peptide (Leinco Prod. No.: A166)
PN:
Acinus (N-Terminus) Blocking Peptide (Leinco Prod. No.: A168)
Acinus (C-Terminus-1) Blocking Peptide (Leinco Prod. No.: A165)
Acinus (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: A167)
Anti-Human Acinus (N-Terminus) (Leinco Prod. No.: A150)
Anti-Human Acinus (C--Terminus 1) (Leinco Prod. No.: A147)
Anti-Human Acinus (Intermediate Domain) (Leinco Prod. No.: A149)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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