Specificity:

Rabbit Anti-Human Amino-terminal Enhancer of Split (AES) recognizes an epitope near the N-terminus of human and mouse AES. This polyclonal antibody was purified using affinity chromatography.

Background:

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment.¹ Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination.^2,3 AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis.⁴ It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family.⁴ Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the α5β1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the α5β1 integrin.⁴

Known Species Cross Reactivity: Ms+, Hu+