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Rabbit Anti-Human Anthrax Toxin Receptor (ATR)
Purified No Carrier Protein
(TEM8)
Polyclonal Antibody
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Prod. No.:A288
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human Anthrax Toxin Receptor (ATR) recognizes an epitope in the intermediate domain of Human, Mouse and Rat ATR. This polyclonal antibody was purified using affinity chromatography.

Background:
The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8).1 This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin.2 Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF).3 This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis.4

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat ATR by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HepG2 Whole Cell Lysate (Leinco Prod. No.: H1404).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Carson-Walter, E. B. et al. (2001) Can. Res. 61:6649
2. Bradley, K. A. et al. (2001) Nature 414:225
3. Molloy, S. et al. (1992) J. Biol. Chem. 267:16396
4. Duesbery, N. et al. (1998) Science 280:734
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HepG2 Whole Cell Lysate (Leinco Prod. No.: H1404)
Anti-Human Anthrax Toxin Receptor (CT) (ATR) (Leinco Prod. No.: A287)
Anthrax Toxin Receptor (CT) Blocking Peptide (Leinco Prod. No.: A311)
Anthrax Toxin Receptor (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: A312)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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