Anti-Human Apoptosis Protease Activating Factor-1 (CT) (Apaf-1) [A156] : Leinco Technologies, excellence in early discovery research

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Rabbit Anti-Human Apoptosis Protease Activating Factor-1 (Apaf-1)

Purified No Carrier Protein

(CED4)

Polyclonal Antibody

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Prod. No.:	A156
Host:	Rabbit
Conc.:	0.5 mg/ml
Pkg. Size:	0.1 mg, 0.5 mg, 1 mg
Storage:	2-8°C Detailed storage instructions below.

Description

Specificity:

Rabbit Anti-Human Apoptosis Protease Activating Factor-1 (Apaf-1) recognizes an epitope near the C-terminus of human, mouse and rat Apaf-1. This polyclonal antibody was purified using affinity chromatography.

Background:

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1.^1,2 Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis.³ Apaf1 can also associate with caspase-4 and caspase-8.⁴ Apaf1 transcript is ubiquitously expressed in human tissues.

Known Species Cross Reactivity: Ms+, Hu+, Rt+

Immunogen

Apoptosis Protease Activating Factor-1 (CT) Blocking Peptide (Leinco Prod. No.: A173)

Blocking Peptide

Apoptosis Protease Activating Factor-1 (CT) Blocking Peptide (Leinco Prod. No.: A173)

Formulation

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

Storage and Stability

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Reported Applications

IHC (NBF/Par.), WB

Each investigator should determine their own optimal working dilution for specific applications.

Country of Origin

USA

References

1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13

2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37

3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89

4. Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (WD1299)