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Rabbit Anti-Human Bfl-1
Purified No Carrier Protein
(Bcl-2-related protein A1, Hemopoietic-specific early response protein)
Polyclonal Antibody
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Prod. No.:B453
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human Bfl-1 recognizes an epitope near the C-terminus of human and mouse Bfl-1. This polyclonal antibody was purified using affinity chromatography.

Background:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumor, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist. Presumably due to post-translational modifications, Bfl-1 is often observed at higher molecular weight in SDS-PAGE than its predicted molecular weight would suggest.

Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Bfl-1 (CT) Blocking Peptide (Leinco Prod. No.: B484)
Blocking Peptide
Bfl-1 (CT) Blocking Peptide (Leinco Prod. No.: B484)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

2. Choi SS, Park IC, Yun JW, et al. A novel Bcl-2 related gene, Bfl-1, is overexpressed in stomach cancer and preferentially expressed in bone marrow. Oncogene 1995; 11:1693-8.

3. Kim JK, Kim KD, Lee E, et al. Up-regulation of Bfl-1/A1 via NF-κB activation in cisplatin-resistant human bladder cancer cell line. Cancer Lett. 2004; 212:61-70.

4. Zhang H, Cowan-Jacob SW, Simonen M, et al. Structural basis of BFL-1 for its interaction with BAX and its anti-apoptotic action in mammalian and yeast cells. J. Biol. Chem. 2000; 275:11092-9
Related Products
Bfl-1 (CT) Blocking Peptide (Leinco Prod. No.: B484)
Anti-Human Bfl-1 (NT) (Leinco Prod. No.: B454)
Bfl-1 (NT) Blocking Peptide (Leinco Prod. No.: B485)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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