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Rabbit Anti-Human CIDE-A
Purified No Carrier Protein
(Cell death-Inducing DFF-like Effector)
Polyclonal Antibody
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Prod. No.:C1292
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human CIDE-A recognizes an epitope near the C-terminus of Human CIDE-A. This polyclonal antibody was purified using affinity chromatography.

Background:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified.1 CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD.1,2 Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-A is expressed in many tissues.

Known Species Cross Reactivity: Hu+
Immunogen
CIDE-A (CT) (Human) Blocking Peptide (Leinco Prod. No.: C1136)
Blocking Peptide
CIDE-A (CT) (Human) Blocking Peptide (Leinco Prod. No.: C1136)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 2.

2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50

3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99

4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6

5. Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
Related Products
CIDE-A (CT) (Human) Blocking Peptide (Leinco Prod. No.: C1136)
Anti-Mouse CIDE-A (CT) (Leinco Prod. No.: C1110)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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