Product Datasheet
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Goat Anti-Human gAcrp30
Biotin
(Adipolean, gAdiponectin)
Polyclonal Antibody
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Prod. No.:A224
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human gAcrp30 recognizes Human gAcrp30. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
gAcrp30 is a naturally occurring globular protein, obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and names AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver.

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human gAcrp30 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody is aseptically filtered and lyophilized from PBS with no carrier proteins or preservatives added.
Reconstitution
Aseptically reconstitute this biotinylated antigen affinity purified polyclonal antibody in sterile 1X PBS containing 0.1% BSA to a final concentration of 50 µg/ml.
Storage and Stability
This lyophilized biotinylated polyclonal antibody should be stored desiccated at -20°C. The reconstituted conjugate is stable for at least one month when stored at 2 - 8°C. For long term storage aseptically aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid More Than One Freeze Thaw Cycle.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Human gAcrp30 this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human gAcrp30 is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.25-1.0 µg/ml when used in conjunction with Anti-Human gAcrp30/Adipolean (Antigen Aff. Pur.) (Leinco Prod. No.: A223) as the capture antibody at 0.5-2.0 µg/ml and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Human gAcrp30. The sensitivity of this sandwich ELISA is 0.2-0.4 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human gAcrp30/Adipolean (Antigen Aff. Pur.) (Leinco Prod. No.: A223)
UltraAvadin™ Reagents
Streptavadin Reagents
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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