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Goat Anti-Human B Cell-Attracting Chemokine-1 (BCA-1)
Biotin
(CXCL13, BLC (mouse), BLR1 Ligand, SCYB13, ANGIE, ANGIE2)
Polyclonal Antibody
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Prod. No.:B116
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human B Cell Attracting Chemokine-1 (BCA-1) recognizes Human BCA-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human BCA-1 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Human BCA-1 this biotin conjugate can be used at a concentration of 0.1 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human BCA-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human BCA-1 (Leinco Prod. No.: B111).
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with Anti-Human BCA-1 (Leinco Prod. No.: C1457) as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Human BCA-1.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human BCA-1 (Leinco Prod. No.: B111)
Anti-Human BCA-1 (Leinco Prod. No.: C1457)
Anti-Mouse BCA-1 (Antigen Aff. Pur.) (Leinco Prod. No.: B574)
Anti-Mouse BCA-1 (Leinco Prod. No.: C1424)
Anti-Mouse BCA-1 (Leinco Prod. No.: C1425)
Anti-Human BCA-1 (Antigen Aff. Pur.) (Leinco Prod. No.: C1480)
Anti-Mouse BCA-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1502)
Recombinant Mouse BLC/BCA-1 (Leinco Prod. No.: B376)
Anti-Human BCA-1 (Antigen Aff. Pur.) (Leinco Prod. No.: B114)
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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