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Rabbit Anti-Human BID
Purified No Carrier Protein
(Caspase-8-Cleaved)
Polyclonal Antibody
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Prod. No.:B456
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human BID recognizes an epitope in the intermediate domain of human and mouse Bid. This polyclonal antibody was purified using affinity chromatography.

Background:
Bid is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bid is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells Bid is cytosolic. In response to Fas ligand or TNF Bid is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane. Cleavage of Bid by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target Bid to the mitochondria. Bid may then interact with another pro-apoptotic Bcl-2 family member Bak. Interaction of Bid with Bak causes altered mitochondrial membrane permeability. A 9 - 13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the Bid interaction with other Bcl-2 family members. Bid is neutralized by binding to the anti-apoptotic member Bcl-x.

Known Species Cross Reactivity: Ms+, Hu+
Immunogen
BID (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B487)
Blocking Peptide
BID (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B487)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Each investigator should determine their own optimal working dilution for specific applications.


References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.

2. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.

3. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.

4. Wang K, Yin XM, Chao DT, et al. BID: a novel BH3 domain-only death agonist. Genes Dev. 1996; 10:2859-69.
Related Products
BID (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B487)
BID (CT) Blocking Peptide (Leinco Prod. No.: B486)
Recombinant Mouse BID (Leinco Prod. No.: B554)
Anti-Mouse BID (Leinco Prod. No.: B594)
Anti-Human/Mouse BID-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B617)
Anti-Human BID (CT) (Leinco Prod. No.: B455)
Recombinant Human BID (Leinco Prod. No.: B550)
Recombinant Human BID (Leinco Prod. No.: B553)
Recombinant Mouse BID (Leinco Prod. No.: B549)
Anti-Human BID ( Anitgen Aff. Pur. ) (Leinco Prod. No.: B644)
Anti-Human/Mouse BID ( Antigen Aff. Pur. ) (Leinco Prod. No.: B645)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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