Product Datasheet
www.leinco.com
excellence in early discovery research       



Rabbit Anti-Human BID (BID)
Antigen Affinity Purified
Polyclonal Antibody
Share |


Prod. No.:B644
Host:Rabbit
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human BID (BID) recognizes Human BID. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human BID (aa 34-52)(>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with ml of sterile 1X PBS pH 7.2-7.3 Containing 0.2% Sodium Azide (NaN3).
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Human BID this polyclonal antibody can be used at a concentration of 1.0 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human BID is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
BID (CT) Blocking Peptide (Leinco Prod. No.: B486)
Recombinant Mouse BID (Leinco Prod. No.: B554)
Anti-Mouse BID (Leinco Prod. No.: B594)
Anti-Human BID (Intermediate Domain) (Leinco Prod. No.: B456)
Anti-Human/Mouse BID-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B617)
Anti-Human BID (CT) (Leinco Prod. No.: B455)
BID (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B487)
Recombinant Human BID (Leinco Prod. No.: B550)
Recombinant Human BID (Leinco Prod. No.: B553)
Recombinant Mouse BID (Leinco Prod. No.: B549)
Anti-Human/Mouse BID ( Antigen Aff. Pur. ) (Leinco Prod. No.: B645)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
359 Consort Drive | St. Louis, MO 63011 | (800) 538-1145 | (636) 527-5545 fax
800.538.1145 ® Copyright Leinco Technologies, Inc. 2006 – 2013