Product Datasheet
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Rabbit Anti-Human β-Actin (CT) (β-Actin (CT))
Purified No Carrier Protein
Polyclonal Antibody
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Prod. No.:B516
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1.0 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human β-Actin recognizes an epitope near the C-terminus of human, mouse and rat β-Actin. This polyclonal antibody was purified using affinity chromatography.

Background:
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism (reviewed in 1). The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging (2). β-actin is one of six different actin isoforms which have been identified. Like GAPDH, β-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
β-actin (CT) Blocking Peptide (Leinco Prod. No.: B502)
Blocking Peptide
β-actin (CT) Blocking Peptide (Leinco Prod. No.: B502)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Lambrechts, A. et al. (2004) Int. J. Biochem. Cell Biol. 36:1890
2. Gourlay, C. W. et al. (2005) Nat. Rev. 6:583
Related Products
β-actin (CT) Blocking Peptide (Leinco Prod. No.: B502)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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