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Rabbit Anti-Human β-Amyloid Precursor Protein (APP)
Purified No Carrier Protein
Polyclonal Antibody
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Prod. No.:A163
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human β-Amyloid Precursor Protein (APP) recognizes an epitope near the C-terminus of Human, Mouse and Rat β-Amyloid Precursor Protein. This polyclonal antibody was purified using affinity chromatography.

Background:
Accumulation of the amyloid-β peptide (αβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The b-amyloid protein precursor (APP) is cleaved by β-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-β peptide (αβ), which is deposited in the brains of all suffers of Alzheimer’s disease.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
β-Amyloid Precursor Protein (CT) Blocking Peptide (Leinco Prod. No.: A281)
Blocking Peptide
β-Amyloid Precursor Protein (CT) Blocking Peptide (Leinco Prod. No.: A281)
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat APP by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Mouse Brain Tissue Lysate (Leinco Prod. No.: M1015).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Ponte, P. et al. (1988) Nature 331:525
2. Selkoe, D. J. (1994) Annu. Rev. Cell Biol. 10:373
Related Products
β-Amyloid Precursor Protein (CT) Blocking Peptide (Leinco Prod. No.: A281)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Mouse Brain Tissue Lysate (Leinco Prod. No.: M1015)
Anti-Human β-Amyloid Precursor Protein (AβNT) (APP) (Leinco Prod. No.: A162)
β-Amyloid Precursor Protein (AβNT) Blocking Peptide (Leinco Prod. No.: A280)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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