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Rabbit Anti-Human CX3CR1
Purified No Carrier Protein
(V28, CMKBRL1)
Polyclonal Antibody
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Prod. No.:C1121
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human CX3CR1 recognizes an epitope near the N-terminus of Human, Mouse and Rat CX3CR1. This polyclonal antibody was purified using affinity chromatography.

Background:
CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively.1-4 The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.2

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), FC, WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 1-2 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human CX3CR1 this polyclonal antibody can be used at a concentration of 0.5-1.0 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Human Spleen Tissue Lysate (Leinco Prod. No.: H1410).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Raport, C. J. et al. (1995) Gene 163:295
2. Combadiere, C. et al. (1995) DNA Cell Biol. 14:673
3. Combadiere. C. et al. (1998) Biochem. Biophys. Res. Commun. 253:728
4. Harrison, J. K. et al. (1994) Neurosci. Lett. 169:85
Related Products
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Human Spleen Tissue Lysate (Leinco Prod. No.: H1410)
Anti-Human CX3CR1 (EL) (Leinco Prod. No.: C1120)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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