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Goat Anti-Human Chemokine (C-C Motif) Ligand 23 (CCL23)
Biotin
(SCYA23, Ckb-8, Myeloid Progenitor Inhibitory Factor-1 (MPIF-1), MIP-3, CKb8, HMRP-2A (Human MIP-Related Protein 2A))
Polyclonal Antibody
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Prod. No.:M196
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human Chemokine (C-C Motif) Ligand 23 (CCL23) recognizes Human CCL23. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Chemokine (C-C motif) ligand 23 (CCL23) is a small cytokine belonging to the CC chemokine family.1 It is primarily expressed in cells of the myeloid lineage.2 CCL23 induces endothelial cell migration and tube formation and enhances the expression of MMP-2 mRNA and protein levels in endothelial cells. CCL23 may play a direct role in angiogenesis.3

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human MIP-3 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Huang SA et al. (2007) J Experimental Hematol. 15: 496
2. Woisetschläger M et al. (2007) J Immunol 178: 4335
3. KIM Jiyoung et al. (2006) Biochemical and Biophysical Res Communications 340: 498

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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