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Goat Anti-Mouse Chemokine (C-C Motif) Ligand 1 (CCL1)
Antigen Affinity Purified
(SCYA1, I-309, T Cell Activation Gene 3 (TCA3), P500, SISe)
Polyclonal Antibody
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Prod. No.:C1483
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Mouse CCL1 recognizes Mouse CCL1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Chemokine (C-C motif) ligand 1 (CCL1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines.1 CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.2 CCL1 may play a role in lymphocyte recruitment in bronchial asthma.3

Immunogen
Sf 21-Derived Recombinant Mouse CCL1
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Mouse CCL1 is approximately 1 ng/well.
Neutralization: This antibody is useful for neutralization of Mouse CCL1 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CCL1 (at 0.01 ng/ml) is 0.3 - 1.5 µg/ml.
Western Blotting: To detect Mouse CCL1 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse CCL1 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Krangel et al. (1992) Proceedings of the National Acad of Sci. 89: 2950
2. Roos et al. (1997) J Biol Chem. 272: 17251
3. Teran LM et al. (2006) Eur Respir J. 28: 59
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CCL1 (Antigen Aff. Pur.) (Leinco Prod. No.: I-231)
Anti-Mouse CCL1 (Leinco Prod. No.: T458)
Anti-Mouse CCL1 (Leinco Prod. No.: T459)
Anti-Mouse CCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: T504)
Anti-Human CCL1 (Leinco Prod. No.: I-643)
Anti-Human CCL1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-177)
Recombinant Human CCL1 (Leinco Prod. No.: I-224)
Recombinant Mouse CCL1 (Leinco Prod. No.: T410)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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