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Goat Anti-Mouse Chemokine (C-X-C Motif) Ligand 10 (CXCL10)
Antigen Affinity Purified
(C7, IFI10, INP10, IP-10, SCYB10, Crg-2, GIP-10, Mob-1)
Polyclonal Antibody
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Prod. No.:I-236
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Mouse Chemokine (C-X-C Motif) Ligand 10 (CXCL10) recognizes Mouse CXCL10. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family. CXCL10 is secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells and fibroblasts.1 CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.2,3 CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury.4

Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse IP-10 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (Frozen), N, WB
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 5-15 µg/ml.
Neutralization: This antibody is useful for neutralization of Mouse CXCL10 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CXCL10 (at 0.5 ug/ml) is 5-25 µg/ml.
Western Blotting: To detect Mouse CXCL10 this polyclonal antibody can be used at a concentration of 1 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Luster et al.(1985) Nature 315: 672
2. Dufour et al.(2002) J. Immunol. 168
3. Angiolillo et al.(1995) J. Exp. Med. 182: 155
4. Kaneko S. et al.(2008) Nephron Exp. Nephrol. 109: c29
Related Products
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CXCL10 (Antigen Aff. Pur.) (Leinco Prod. No.: I-160)
Anti-Cotton Rat CXCL10 (Antigen Aff. Pur.) (Leinco Prod. No.: I-601)
Anti-Human CXCL10 (Leinco Prod. No.: C1389)
Anti-Mouse CXCL10 (Leinco Prod. No.: C1423)
Anti-Human CXCL10-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-275)
Anti-Mouse CXCL10-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: I-276)
Human CXCL10 ELISA Development Kit (Leinco Prod. No.: I-423)
Recombinant Human CXCL10 (Leinco Prod. No.: I-202)
Recombinant Mouse IP-10 (Leinco Prod. No.: I-229)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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