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Goat Anti-Mouse Chemokine (C-X-C Motif) Ligand 2 (CXCL2)
Biotin
(SCYB2, GRO2, Growth-Regulated Protein Beta (GROb), MIP-2a, MGSA-b, CINC-2a, MIP-2, Cytokine-Induced Neutrophil Chemotactic Factor 3 (CINC-3))
Polyclonal Antibody
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Prod. No.:M195
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Mouse Chemokine (C-X-C Motif) Ligand 2 (CXCL2) recognizes Mouse CXCL2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Chemokine (C-X-C motif) ligand 2 (CXCL2) is an inducible murine chemokine involved in attraction of polymorphonuclear granulocytes to sites of infection.1 This chemokine is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells.2,3,4

Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse CXCL2 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, IHC (NBF/Par.), WB,
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 - 15 µg/ml. The recommended secondary antibody for IHC is UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Mouse CXCL2 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse CXCL2 is 1.5-3.0 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse MIP-2 (Leinco Prod. No.: M174).
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with Anti-Mouse CXCL2 (Leinco Prod. No.: M1277) as the capture antibody at 2 µg/ml and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Mouse CXCL2.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. H. Ulrich Beuscher et al. (2004) International Immunol. 16: 1675
2. Cerami A et al. (1989) Proc Nat Acad Sci. 86: 612
3. Grotendorst G.R et al. (1990) Mol Cell Biol. 10: 5596
4. Fukuda S et al. (2006) Exp Hematol. 34: 1010
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse MIP-2 (Leinco Prod. No.: M174)
Anti-Mouse CXCL2 (Leinco Prod. No.: M1277)
Anti-Human CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G146)
Anti-Human CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G148)
Anti-Rat CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: G122)
Anti-Mouse CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: M178)
Anti-Human CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: G133)
Mouse CXCL2 ELISA Development Kit (Leinco Prod. No.: M1108)
Recombinant Mouse CXCL2 (Leinco Prod. No.: G632)
Anti-Human CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: G136)
Human GROβ (CXCL2) ELISA Development Kit (Leinco Prod. No.: G595)
Recombinant Rat CXCL2 (Leinco Prod. No.: C1343)
Anti-Rat CXCL2 (Antigen Aff. Pur.) (Leinco Prod. No.: C1465)
Anti-Rat CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1509)
Anti-Rat CXCL2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G147)
Anti-Rat CXCL2 (Leinco Prod. No.: C1433)
Recombinant Human CXCL2 (Leinco Prod. No.: G131)
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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