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Goat Anti-Mouse Chemokine (C-X-C Motif) Ligand 7 (CXCL7)
Biotin
(PPBP, PBP, B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, LA-PF4, LDGF, MDGF, Neutrophil Activating Peptide 2 (NAP-2), SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1)
Polyclonal Antibody
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Prod. No.:C1523
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Mouse CXCL7 recognizes Mouse CXCL7. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
CXC chemokine ligand 7 is a small cytokine belonging to the CXC chemokine family. It is a protein that is released in large amounts from platelets following their activation.1 It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator.2,3

Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse CXCL7 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, IHC (NBF/Par.), WB,
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5-15 µg/ml. The recommended secondary antibody for IHC is UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Mouse CXCL7 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse CXCL7 is 0.5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 200 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Poncz M et al. (1991) J Biol Chem. 266: 5785
2. Walz D et al. (1983) Proc Natl Acad Sci USA 80: 765
3. Carter-Su C et al. (1985) Biochemistry 24: 1762
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CXCL7 (Antigen Aff. Pur.) (Leinco Prod. No.: N112)
Recombinant Mouse CXCL7 (Leinco Prod. No.: T380)
Recombinant Rat CXCL7 (Leinco Prod. No.: T381)
Anti-Mouse CXCL7 (Leinco Prod. No.: C1380)
Anti-Mouse CXCL7 (Antigen Aff. Pur.) (Leinco Prod. No.: C1478)
Anti-Human CXCL7-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: N124)
Anti-Human CXCL7 (Leinco Prod. No.: N139)
Recombinant Mouse Thymus Chemokine-1 (Leinco Prod. No.: T530)
UltraAvadin™ Reagents
Streptavadin Reagents
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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