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Rabbit Anti-Human Collapsin Response Mediator Protein 1 (CRMP1)
Purified No Carrier Protein
(DRP-1, Dihydropyropyrimidinase-Related Protein 1)
Polyclonal Antibody
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Prod. No.:C1300
Host:Rabbit
Conc.:0.5 mg/ml
Pkg. Size:0.1 mg, 0.5 mg, 1 mg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Rabbit Anti-Human Collapsin Response Mediator Protein 1 (CRMP1) recognizes an epitope in the intermediate domain of Human and Mouse CRMP1. This polyclonal antibody was purified using affinity chromatography.

Background:
Collapsin-response mediator proteins (CRMPs) are highly expressed in the developing brain where they play major roles in axonal outgrowth, neurite differentiation, and apoptosis.1 Their continued expression in areas of high synaptic remodeling such as the cerebellum, hippocampus, and the olfactory system suggests that these proteins may also be involved in adult brain plasticity.2 CRMP-1 was initially identified as a dihydro-pyrimidinase expressed exclusively in brain3; later studies have shown that it is involved with neurotrophin (NT) 3-induced neurite formation and outgrowth.4 CRMP-1 localization switches from axonal to somatodendritic when neurons reach functional maturity, suggesting that it is involved in early neuronal differentiation as well as in later processes related to the survival or death of the newly generated neurons.

Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Collapsin Response Mediator Protein 1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1303)
Blocking Peptide
Collapsin Response Mediator Protein 1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1303)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human CRMP1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Caco-2 Whole Cell Lysate (Leinco Prod. No.: C1182).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Charrier, E. et al. (2003) Mol. Neurobiol. 28:51
2. Cameron, H. A. et al. (2001) J. Comp. Neurol. 435:406
3. Hamajima, N. et al. (1996) Gene 180:157
4. Quach, T. T. et al. (2004) Mol. Cell. Neurosci. 25:433
Related Products
Collapsin Response Mediator Protein 1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: C1303)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Caco-2 Whole Cell Lysate (Leinco Prod. No.: C1182)
Anti-Human Collapsin Response Mediator Protein 1 (CT) (CRMP1) (Leinco Prod. No.: C1299)
Collapsin Response Mediator Protein 1 (CT) Blocking Peptide (Leinco Prod. No.: C1302)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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