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Goat Anti-Human ErbB3
Antigen Affinity Purified
(V-erb-b2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian), ERBB3, HER3, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3)
Polyclonal Antibody
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Prod. No.:E231
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human ErbB3 recognizes Human ErbB3. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian), also known as ERBB3 is a member of the epidermal growth factor (EGF) receptor subfamily of receptor tyrosine kinases and is believed to be a receptor for an unknown ligand.1 ErbB3 has a heregulin (HRG) or neuregulin binding domain but lacks intrinsic protein tyrosine kinase activity.2 Therefore, it can bind to the ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity.
Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of ErbB3 and/or over-expression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors.

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human ErbB3 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1.0 µl of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, FC, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human ErbB3 is approximately 0.2 ng/well.
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing ErbB3. A suggested method would be to stain cells expressing ErbB3 with 0.50 µg per 1-2.5 x 105 cells in a total staining volume of ≤200 µl followed by Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641).
Neutralization: This antibody is useful for neutralization of Human ErbB3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human ErbB3 (at 10 ng/ml) is 0.125-0.5 µg/ml.
Western Blotting: To detect Human ErbB3 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human ErbB3 is 1 ng/lane under non-reducing and 10 ng/lane under reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human ErbB3 (Leinco Prod. No.: E197).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Cerione RA. et al. (1994) J Biol Chem. 269: 14303
2. Koland JG. et al. (1998) Biochem J. 333: 757
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
Donkey F(ab')2 Anti-Goat IgG (H&L)-R-phycoerythrin (R-PE) (Leinco Prod. No.: G641)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human ErbB3 (Leinco Prod. No.: E197)
Anti-Human ErbB3-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: E275)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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