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Goat Anti-Human Fibroblast Growth Factor-6 (FGF-6)
Antigen Affinity Purified
(HBGF-6, HST-2)
Polyclonal Antibody
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Prod. No.:F1059
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human Fibroblast Growth Factor 6 (FGF-6) recognizes Human FGF-6. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Fibroblast growth factor 6, also known as FGF6, hst-2 or hst-1-related is a member of the fibroblast growth factor (FGF) family. 1,2 FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF6 is the only known member of the FGF family whose expression is restricted to the muscle cell lineage during development, suggesting it may have a role in myogenesis.3 FGF6 has a dual function in muscle regeneration, stimulating myoblast proliferation/migration and muscle differentiation/hypertrophy.4

Known Species Cross Reactivity: Hu+
Immunogen
Purified Recombinant Human FGF-6 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1.0 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<10 ng/mg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human FGF-6 is approximately 0.16 ng/well.
Neutralization: This antibody is useful for neutralization of Human FGF-6 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human FGF-6 (at 1.0 ng/ml of FGF-6 and 1.0 µg/ml of heparin) is 0.025-0.075 µg/ml.
Western Blotting: To detect Human FGF-6 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human FGF-6 is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human FGF-6 (Leinco Prod. No.: F121).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Iida S et al. (1992) Oncogene 7: 303
2. Marics I et al. (1989) Oncogene 4: 335
3. DeLapeyrière O. et al. (1996) Exp Cell Res. 224: 143
4. Chanoine C. et al. (2006) Biochim Biophys Acta 1763: 773
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human FGF-6 (Leinco Prod. No.: F121)
Anti-Human FGF-6-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: F1092)
Anti-Human FGF-6 (Leinco Prod. No.: F1067)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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