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Goat Anti-Human Fibroblast Growth Factor-Basic (FGF-Basic)
Biotin
(B-FGF, FGF-2, FGF-β, FGFB, Prostatropin, NUDT6)
Polyclonal Antibody
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Prod. No.:F134
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human Fibroblast Growth Factor-Basic (FGF-Basic) recognizes Human FGF-Basic. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Acidic and basic fibroblast growth factors (FGFs) are members of a family of proteins that exert pleiotropic effects in a range of cell types including skeletal myocytes.1 Fibroblast growth factor basic (FGF basic) significantly promote angiogenesis; a central event for the growth and metastasis of solid tumors.2 It is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland, liver, placenta, and monocytes, epithelial and endothelial cells. FGF-basic plays a central role during prenatal development and postnatal growth and regeneration of tissues, by promoting cellular proliferation and differentiation. FGF-basic is one of the angiogenesis factors and may promote wound healing.3

Known Species Cross Reactivity: Hu+
Immunogen
E. coli-Derived Recombinant Human FGF Basic
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
IHC (NBF/Par.), WB
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 5 µg/ml. The recommended secondary antibody for IHC is UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human FGF-Basic this biotin conjugate can be used at a concentration of 0.1-0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human FGF-Basic is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Human FGF Basic (Leinco Prod. No.: F1105).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Swain JL et al. (1991) Developement 111: 741
2. Grevers G et al. (1997) Laryngorhinootologie 76: 421
3. (2007) ORG Genetics
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Human FGF Basic (Leinco Prod. No.: F1105)
Anti-Human FGF-Basic (Antigen Aff. Pur.) (Leinco Prod. No.: F107)
Recombinant Mouse FGF-Basic (Leinco Prod. No.: F1022)
Recombinant Rat FGF-Basic (Leinco Prod. No.: F1027)
Human FGF-Basic ELISA Development Kit (Leinco Prod. No.: F167)
Recombinant Human FGF-Basic (Leinco Prod. No.: F110)
Anti-Human FGF-Basic (Leinco Prod. No.: F149)
UltraAvadin™ Reagents
Streptavadin Reagents
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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