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Goat Anti-Human Fractalkine (FKN)
Biotin
(CX3CL1, NTN, ABCD-3, C3Xkine, CXC3, CXC3C, NTT, SCYD1, Neurotactin, FKN)
Polyclonal Antibody
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Prod. No.:F136
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human Fractalkine recognizes Human Fractalkine. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Fractalkine (FKN) is a transmembrane mucin-chemokine hybrid molecule expressed on activated endothelium1 that mediates attachment and firm adhesion of T cells, monocytes and NK cells.2 FKN plays an important proinflammatory role in rheumatoid arthritis (RA) pathogenesis as characterized by induction of synovial angiogenesis, chemotaxis, activation of monocytes and T cells as well as the stimulation of proliferation and synthesis of matrix degrading enzymes (matrix metalloproteinases, MMP) in synovial fibroblasts. Fractalkine thus may represent a novel target molecule for therapeutic intervention in RA.3 FKN is also an essential biomarker for predicting the prognosis of patients with colorectal cancer (CRC).4

Immunogen
Purified Recombinant Human Fractalkine (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
ELISA Det, WB,
Western Blotting: To detect Human Fractalkine this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human Fractalkine is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 200 µg/ml when used in conjunction with Anti-Human Fractalkine (Antigen Aff. Pur.) (Leinco Prod. No.: F120) as the capture antibody at 100 µl/well and an optimal second step reagent such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) for the detection of Human Fractalkine. The sensitivity of this sandwich ELISA is 5 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Patel DD et al. (1998) J Exp Med. 188: 1413
2. Hwang ST et al. (1999) Eur J Immunol. 29: 2551
3. Muller GA et al. (2008) Z Rheumatol. 67: 424
4. Mori M et al. (2005) Int J Oncol. 26: 41
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human Fractalkine (Antigen Aff. Pur.) (Leinco Prod. No.: F120)
Anti-Human Fractalkine (Leinco Prod. No.: C1306)
Recombinant Rat Fractalkine (Leinco Prod. No.: F1023)
Recombinant Human Fractalkine (CX3CL1) (Leinco Prod. No.: F1030)
Recombinant Mouse Fractalkine (Leinco Prod. No.: F1035)
Recombinant Mouse Fractalkine (Leinco Prod. No.: F1036)
Recombinant Rat Fractalkine (Chemokine Domain, aa 25-100) (Leinco Prod. No.: F1038)
Recombinant Mouse Fractalkine (Fragmented) (Leinco Prod. No.: F1040)
Anti-Human Fractalkine (Leinco Prod. No.: C1405)
Anti-Human Fractalkine (Leinco Prod. No.: C1406)
Anti-Rat Fractalkine (Leinco Prod. No.: C1438)
Anti-Mouse Fractalkine (Leinco Prod. No.: C1442)
Anti-Rat Fractalkine (Antigen Aff. Pur.) (Leinco Prod. No.: C1467)
Anti-Human Fractalkine-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1492)
Anti-Rat Fractalkine-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1512)
Anti-Mouse Fractalkine (Antigen Aff. Pur.) (Leinco Prod. No.: F1069)
Recombinant Human Fractalkine (CX3CL1) (Leinco Prod. No.: F116)
Recombinant Rat Fractalkine (Leinco Prod. No.: F1115)
Anti-Mouse Fractalkine-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: F1098)
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