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Goat Anti-Human/Mouse Glial Cell Line-Derived Neurotropic Factor Receptor Alpha 2 (GFRα-2)
Antigen Affinity Purified
(GDNFRB, NRTNR-Alpha, NTNRA, RETL2, TRNR2)
Polyclonal Antibody
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Prod. No.:G660
Host:Goat
Pkg. Size:100 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Human/Mouse Glial Cell-Line Derived Neurotrophic Factor Receptor Alpha 2 (GFRα-2) recognizes Human/Mouse GFRα-2. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Purified Recombinant Human/Mouse GFRα-2 (>98%)
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this lyophilized, antigen affinity purified polyclonal antibody with 1.0 ml of sterile 1X PBS pH 7.2-7.3. This will result in a final antibody concentration of 0.1 mg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), WB, B
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). The detection sensitivity of this indirect ELISA for Human/Mouse GFRα-2 is approximately 1.5 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2-15 µg/ml. The recommended secondary antibody for IHC is Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505). For chromogenic detection with high signal and low background use 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Western Blotting: To detect Human/Mouse GFRα-2 this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human/Mouse GFRα-2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Blocking: Blockade of receptor-ligand interaction - Approximately 1-3 μg/ml of this antibody will block 50% of the binding of 2 ng/ml recombinant human GDNF to immobilized recombinant mouse GFRα-2/Fc Chimera (100 μl of a 1 μg/ml solution was coated in each well) in a functional ELISA assay.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Lewin GR et al. (2002) J Physiol. 545: 43
2. Mulligan Lois M et al. (2001) Human Genetics 108: 409
3. Herzig KH et al. (2005) Diabetes 54: 1324
Related Products
Donkey Anti-Goat IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: G505)
3, 3'-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100)
Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
GFRα-2 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: G624)
Recombinant Mouse GFRα-2 (Leinco Prod. No.: G646)
Recombinant Human GFRα-2 (Leinco Prod. No.: G649)
Anti-Human GFRα-2 (Antigen Aff. Pur.) (Leinco Prod. No.: G683)
Anti-Mouse GFRα-2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G689)
Anti-Human GFRα2-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: G693)
Anti-Human GFRα-2 (Intermediate Domain) (Leinco Prod. No.: G577)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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